WebOct 13, 2024 · The human cytomegalovirus protein US11 induces the dislocation of MHC class I heavy chains from the endoplasmic reticulum (ER) into the cytosol for degradation by the proteasome. With the use of a fractionated, permeabilized cell system, we find that US11 activity is needed only in the cell membranes and that additional cytosolic factors are … WebAug 28, 2024 · Two solutions (1.7 mL each) of 35% and 5% sucrose were laid sequentially on the top of the 45% sucrose solution. After ultracentrifugation at 39,000 rpm in a Beckman Optima TM L-70K centrifuge using a SW 40 Ti rotor for 20 h, 11 individual 0.5-mL fractions were collected from the top of the tubes, and 50 µL of each fraction were analyzed by …
Capítol 2. El VHE a l’ambient.
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Instructions For Use - Biomolecular Chemistry
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