WebFeb 20, 2015 · High affinity RNA ligands to human nerve growth factor (NGF) were selected from pools of random RNA using SELEX [Tuerk, C. and Gold, L. (1990) Science, 249, 505-510]. WebApr 8, 2010 · Tuerk, C.; Gold, L. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science 1990 , 249 , 505–510. [ Google Scholar ]
Multivalent Sgc8c-aptamer decorated polymer scaffolds for leukemia ...
WebMar 18, 1996 · McGee, D.P.C.; Settle, A.; Vargeese, C.; Zhai, Y. J. Org. Chem. in press. 16. During the DBU catalyzed cyclization of OTBDMS derivative 12, a minor amount (9% isolated yield) of a cyclization product resulting from nucleophilic attack by the carbamate carbonyl oxygen was formed, as was a significant amount of N-O desilylated product (36%). WebDec 17, 2024 · Aptamers are attractive constructs due to their high affinity/selectivity towards a target. Here 7,8-dihydro-8-oxoguanosine (8-oxoG) has been used, due in part to its unique H-bonding capabilities (Watson–Crick or Hoogsteen), to expand the … high thermal conductivity liquid
A wearable electrochemical fabric for cytokine monitoring
WebTo select aptamers with modified nucleotides, the appropriate DNA polymerase must be designated.We studied eight polymerases and demonstrated that incorporating a tryptophan-like nucleotide into aptamer libraries by emulsion PCR and KOD XL had an insignificant effect on the PCR bias and did not distort the sequence space, thus making … WebOct 1, 2007 · Nature 346, 818–822; Tuerk, C., Gold, L., 1990. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. … WebApr 9, 2024 · Compared with commonly used antibodies, aptamer, a class of functional oligonucleotides engineered by artificial combinatorial methodologies, exhibits better chemical and thermal stability, lower synthetic cost, and high affinity for all molecules theoretically (Tuerk and Gold, 1990; X. Wang et al., 2024; WilsonSzostak, 1999). how many different types of pneumonia