WebIn this work we establish the activity and substrate preference for six MTs from the gephyronic acid polyketide synthase and demonstrate their ability to methylate both N-acetylcysteamine- and acyl carrier protein-linked β … WebAug 29, 2024 · Polyketide synthases (PKS) are a rich source of natural products of varied chemical composition and biological significance. Here, we report the characterization of an atypical dehydratase (DH) domain from the PKS pathway for gephyronic acid, an inhibitor of eukaryotic protein synthesis. Using a library of synthetic substrate mimics, the ...

Elucidation of Gephyronic Acid Biosynthetic Pathway …

Gephyronic acid is a polyketide that exists as an equilibrating mixture of structural isomers. In nature, gephyronic acid is produced by slow growing myxobacterium: Archangium gephyra strain Ar3895 and Cystobacter violaceus strain Cb vi76. It is the first antibiotic in myxobacteria that was reported … See more Preliminary studies demonstrated that gephyronic acid inhibited growth of yeast and molds as well as elicited a cytostatic effect through the inhibition of eukaryotic protein synthesis in mammalian cell cultures. Feeding … See more Sequencing of the PKS gene cluster in C. violaceus was conducted and confirmed to reveal five type I polyketide synthases and post-PKS tailoring enzymes with O-methyltransferase and cytochrome P450 monooxygenase. The overall structure correlates well with … See more WebDec 12, 2024 · Isotopic labeling of the gephyronic acid producer Cystobacter violaceus with 13 C-methyl-L-methionine demonstrated that the isobutyryl starter group is derived from S-adenosylmethionine (SAM) and not from valine, likely via GphF MT1 18. The MT1-containing loading modules also include a mysterious ~150-residue N-terminal “adaptor … marvin upshaw

Repurposing the GNAT Fold in the Initiation of ... - ScienceDirect

WebFeb 8, 2010 · Richard Taylor from the Department of Chemistry and Biochemistry – to identify the key steps in the biosynthesis of gephyronic acid, a natural product isolated from a bacteria that has been shown to inhibit growth of yeast and mold, as well as a range of mammalian cell types. Identification of the gene cluster responsible for biosynthesis ... WebCoupled to the development of a total synthesis of gephyronic acid, a series of diastereomeric analogues and their precursors have been prepared by employing complementary aldol strategies for the ... WebCoupled to the development of a total synthesis of gephyronic acid, a series of … hunting tattoos designs