WebOR3R: 3515.0: 0819: 03/05/2024: NO EARS ON 081: 06/2024 - 03/2024 Spot Statistics # of Spots: 0 # times Spotted: 3: Spots by Time Of Day The spot counts both spottee & spotter. The total values reflect the # of events the Callsign showed up … WebOR3R, 5'-ACGACATGCTTATGCCTCTC-3', PCR amplification was performed with the following conditions: a denaturing step at 95 °C for 5 min, followed by 35 cycles (95 °C for 30 s, 55 °C for 30 s, 72 °C for 30 s), and a final step of 7 min at 72 °C. The 200-bp PCR product was purified and ligated into a pUC 19-T vector (Takara, China).
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