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Stat3 chip

WebChIP Instrument model Illumina Genome Analyzer II Description ChIP against STAT3 Data processing For ChIP-Seq and RNA-Seq, Basecalls performed using CASAVA 1.8 Raw reads were aligned to the mm9 genome assembly using Bowtie 0.12.9 Aligned reads are converted to BED format and removed redundancies using unpublished python and perl scripts WebMar 21, 2024 · STAT3 (Signal Transducer And Activator Of Transcription 3) is a Protein Coding gene. Diseases associated with STAT3 include Autoimmune Disease, Multisystem, Infantile-Onset, 1 and Hyper-Ige Recurrent Infection Syndrome 1, Autosomal Dominant . Among its related pathways are IL-9 Signaling Pathways and Prolactin Signaling .

Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb #9145

WebThe present specification discloses a novel use of granulocyte colony-stimulating factor receptor or colony-stimulating factor 3 receptor (CSF3R) as a biomarker and as a therapeutic target for pulmonary fibrosis. The present specification discloses a treatment mechanism, a treatment method, and a treatment drug involving CSF3R for pulmonary fibrosis, and a … WebAug 31, 2024 · Such YAP/TAZ sites with only a single motif strongly suggest the existence of an AP-1/STAT3/TEAD complex. ChIP-seq experiments do not formally exclude the possibility that AP-1, STAT3, and TEAD occupy target regions at different times. However, this seems very unlikely at sites with only a single motif because it is hard to imagine how … smaller rotations in tabletop simulator https://rooftecservices.com

Stat3 Antibody Cell Signaling Technology

WebJun 11, 2024 · STAT3 and GR cooperate to drive basal-like triple negative breast cancer gene expression and proliferation. Breast cancers can be divided into subtypes with … WebAn HRP-conjugated secondary antibody provides sensitive colorimetric readout that at OD 450 nm. This product detects human, mouse and rat STAT3. Key performance and benefits: Assay time: 3.5 hours (cell extracts preparation not included). Detection limit: < 0.6 µg nuclear extract/well. Detection range: 0.3 – 10 µg nuclear extract/well. small error probability

A STAT3-dependent transcriptional circuitry inhibits cytotoxic ... - PNAS

Category:Understanding STAT3, the intersection of many …

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Stat3 chip

STAT3 gene: MedlinePlus Genetics

WebMar 8, 2024 · STAT3 ChIP-seq revealed a mapping of 611 and 2,289 differential STAT3-binding sites in human B cells after TLR7 and TLR9 agonists stimulation, respectively. … WebCancers. STAT3 gene mutations are found in approximately one-third of cases of a blood cancer called large granular lymphocytic leukemia (LGL), which is characterized by the …

Stat3 chip

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WebMar 1, 2011 · STAT3 inhibition of HT29 recognition by NK cells involves a TGF-β1–independent mechanism NK functions result from the integration of activating and inhibitory signaling related to different ligands recognized on target cells. WebMonoclonal Antibody for studying STAT3. Cited in 1669 publications. Validated for WB, WB, IP, IHC, IF, F, ChIP, C&amp;R. Available in 2 sizes. Highly specific and rigorously validated in-house, Stat3 (124H6) Mouse Monoclonal Antibody (CST #9139) is ready to ship.

WebFeb 2, 2008 · Activation of the transcription factor STAT3 is thought to potently promote oncogenesis in a variety of tissues, leading to intense efforts to develop STAT3 inhibitors for many tumors, including the highly malignant brain tumor glioblastoma. ... ChIP analysis at the endogenous LIFRβ promoter in Pten loxP/loxP and Pten −/ ... WebNov 27, 2024 · STAT3-induced RORγt represses cytotoxic genes by inhibiting the functions but not the expression of the “cytotoxic” transcription factors T-bet and Eomesodermin. Thus, the transcriptional circuitry directing IL-17 expression inhibits cytotoxic functions.

WebSTAT3 associates with Glut5 promoter and activates Glut5 transcription in response to IL-6. (A-B) DU145 and HSC-3 cells were stimulated with IL-6 (50 ng/mL) for the indicated periods of time. WebChromatin immunoprecipitations were performed with cross-linked chromatin from Hep G2 cells starved overnight and treated with Human Interleukin-6 (hIL-6) #8904 (100 ng/ml) for 30 minutes and Stat3 (D3Z2G) Rabbit mAb, using SimpleChIP ® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit …

WebDot: STAT3 phospho Y705 peptide (aa700-710) ChIP: HepG2 (starved overnight) treated with IL-6. General notes This product is a recombinant monoclonal antibody, which offers …

WebAug 2, 2024 · Human STAT3, His Tag (Cat. No. ST3-H5149) immobilized on CM5 Chip can bind STAT3 inhibitor with an affinity constant of 44.6 nM as determined in a SPR assay (Biacore 8K) (QC tested). smaller scholars montessori grisbyWebJun 12, 2024 · STAT3 is a transcription factor regulating genes for cell survival, proliferation and metabolism [ 13, 14 ]. Interestingly, STAT3 can also enter the mitochondria to … song have thine own way lord by female singerWebApr 13, 2024 · Chromatin immunoprecipitation sequencing (ChIP-seq) in the progenitor cell line HPC7 expressing STAT3,STAT3 Y640F, or empty vector as control was used to understand whether mutant STAT3 alters chromatin occupancy. Our aim was to understand which of the 129 genes required in mutant cells are direct STAT3 targets. smaller royal caribbean shipsWebIdentification of Novel Direct Stat3 Target Genes–The majority of the Stat3 target genes isolated by ChIP using NIH3T3 cells with a 30-min OSM stimulation have not been identified previously by microarray analyses. To confirm that we have identified novel direct Stat3 target genes, gene-specific ChIP analyses were further performed on these ... smaller scholars montessoriWebApr 25, 2024 · The subsequent ChIP experiments reveal that Stat3 directly binds to the promoter of the late osteogenic marker osteocalcin (OCN) and further enhances this effect upon the incorporation of Runx2 (Xu et al., 2024). Simultaneously, the synergistic effect of Stat3 and Runx2 on OCN promoter activity could be reversed by AG490 (Xu et al., 2024). … smaller scholars 77077WebApr 13, 2024 · Upon activation, STAT3 proteins are tyrosine-phosphorylated (pY 705 STAT3) by JAK, dimerize, and translocate to the nucleus where they regulate transcription. 12 … song have thine own way lordWebDec 6, 2024 · STAT3 regulates IgE class switching in a Th2-type response. STAT3 f/f control or CD2-Cre; STAT3 f/f mice were immunized i.p. with 100 μg OVA in Alum. ( A) Sera were collected at indicated time points and OVA Ab levels of different isotypes (IgE, IgG, and IgG 1) were detected by ELISA. Data are means ± SEM. smaller scholars montessori academy tuition